A lower TRα diversity was observed in recipients of a cytomegalovirus-seropositive donor ( P=0.014). Although there was a remarkable expansion of single clones observed in the recipients’ memory CD8 + TRα repertoire, no clear association between graft- versus-host disease or cytomegalovirus infection and T-cell receptor diversity was identified. When comparing donor and recipient, approximately 50% and approximately 80% of the donors’ memory repertoire were later retrieved in the naïve and memory CD8 + T-cell receptor repertoire of the recipients, respectively. Sequencing of the TRα identified a repertoire consisting of more dominant clonotypes (>1% of reads) in these patients at 6 and 18 months post transplantation. When comparing the influence of anti-T-cell therapy, a delay in the reconstitution of the naïve CD8 + T-cell repertoire was observed in patients who received in vivo T-cell depletion using antithymocyte globulin or post-transplantation cyclophosphamide in case of haploidentical transplantation. In parallel, reconstitution of the CD8 +/CD4 + T-cell subsets was mapped using flow cytometry.
Using next-generation sequencing, the T-cell receptor alpha (TRα) repertoire of naïve and memory CD8 + T cells from 25 patients who had received different forms of allogeneic transplantation was analyzed. How these immune reactions result in changes to the T-cell receptor repertoire remains largely unknown.
Alloreactivity or opportunistic infections following allogeneic stem cell transplantation are difficult to predict and contribute to post-transplantation mortality.
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